EXPLORATION OF INDIGENOUS ARBUSCULAR MYCORRHIZAL FUNGI ON ARENGA PINNATA MERR IN POST-MINING LAND

Shaleha Istiqamah Samal; Irdika Mansur; Ahmad Junaedi

doi:10.30556/imj.Vol26.No1.2023.1285

Authors

Shaleha Istiqamah Samal
Irdika Mansur IPB University
Ahmad Junaedi IPB University

DOI:

https://doi.org/10.30556/imj.Vol26.No1.2023.1285

Keywords:

indigenous AMF, revegetation, root colonization, spores, sugar palm plant

Abstract

Mining activities have a positive impact that can generate income for the state, but the activities also cause negative impacts in the form of soil damage, vegetation and animal losses to disrupt the ecosystems. Therefore, it is necessary to carry out a reclamation with revegetation using local plant species. PT Berau Coal has conducted revegetation using a local sugar palm plant (Arenga pinnata). The success of sugar palm plant growth in post-mining reclamation land is influenced by several factors, one of it is the symbiosis of the arbuscula mycorrhizal fungi (AMF) with the sugar palm planted by PT Berau Coal. The purpose of this study was to determine the diversity of indigenous spores in the arenga rhizosphere and root colonization of sugar palm plants. Identification of the AMF diversity was carried out by observing the soil taken from the palm rhizosphere with a depth of 0-20 cm and 20-40 cm. Isolation of spores using the wet pouring technique method with centrifuges and AMF spores were identified using the INVAM methods. The observation of AMF colonization on plant roots was carried out through the root staining technique with the Clapp modification method. The results showed that the AMF spores were found in 3 AMF genera at the observation site, namely genus Glomus (15 sp), Acaulospora (3 sp), and Gigaspora (1 sp). The highest spore abundance is genus Glomus sp at a soil depth of 0-20 cm. The AMF structures found colonizing the roots of sugar palm plants are hyphae, vesicles, and spores.

References

Agus, C., Pradipa, E., Wulandari, D., Supriyo, H., Saridi and Herika, D. (2014) ‘Peran revegetasi tanah pada lahan rehabilitasi tambang batubara di daerah tropika’, Jurnal Manusia dan Lingkungan, 21(1), pp. 60–66.

Agustin, W. (2011) Inokulasi fungi mikoriza arbuskula untuk meningkatkan produktivitas dan mutu benih cabai (Capsicum annum L) serta efisiensi penggunaan pupuk P. Institut Pertanian Bogor.

Birhane, E., Gebremedihin, K.M., Tadesse, T., Hailemariam, M. and Solomon, N. (2017) ‘Exclosures restored the density and root colonization of arbuscular mycorrhizal fungi in Tigray, Northern Ethiopia’, Ecological Processes, 6(1), p. 33. Available at: https://doi.org/10.1186/s13717-017-0101-9.

Ervayenri (2020) ‘Keanekaragaman fungi mikoriza arbuskula (FMA) lahan Kampus Universitas Lancang Kuning (UNILAK)’, Wahana Forestra: Jurnal Kehutanan, 15(2), pp. 79–92. Available at: https://doi.org/10.31849/forestra.v15i2.5122.

Hadianur, Syafruddin and Kesumawati, E. (2016) ‘Pengaruh jenis fungi mikoriza arbuscular terhadap pertumbuhan dan hasil tanaman tomat (Lycopersicum esculentum Mill)’, Jurnal Agrista, 20(3), pp. 126–134.

Herlina, A., Handayani, H.E. and Iskandar, H. (2014) ‘Pengaruh fly ash dan kapur tohor pada netralisasi air asam tambang terhadap kualitas air asam tambang (pH, Fe & Mn) di IUP Tambang Air Laya PT. Bukit Asam (Persero),Tbk’, Jurnal Ilmu Teknik, 2(2).

Hermawan, H., Muin, A. and Wulandari, R.S. (2015) ‘Kelimpahan fungi mikoriza arbuskula (FMA) pada tegakan ekaliptus (Eucalyptus pellita) berdasarkan tingkat kedalaman di lahan gambut’, Jurnal Hutan Lestari, 3(1), pp. 124–132.

Kurnia, Gusmiaty and Larekeng, S.H. (2019) ‘Identifikasi dan karakterisasi mikoriza pada tegakan nyatoh (Palaquium sp.)’, PERENNIAL, 15(1), pp. 51–57. Available at: https://doi.org/10.24259/perennial.v15i1.6850.

Lamit, L.J., Romanowicz, K.J., Potvin, L.R., Rivers, A.R., Singh, K., Lennon, J.T., Tringe, S.G., Kane, E.S. and Lilleskov, E.A. (2017) ‘Patterns and drivers of fungal community depth stratification in Sphagnum peat’, FEMS Microbiology Ecology, 93(7). Available at: https://doi.org/10.1093/femsec/fix082.

Liu, R.-J., Li, Y., Diao, Z.-K., Li, M. and Lin, X.-G. (2013) ‘Effects of soil depth and season variation on community structure of Arbuscular Mycorrhizal Fungi in Greenhouse Soils Planted with Watermelon’, Pedosphere, 23(3), pp. 350–358. Available at: https://doi.org/10.1016/S1002-0160(13)60026-0.

Menteri Energi dan Sumber Daya Mineral (2018) Peraturan Menteri Energi dan Sumber Daya Mineral Republik Indonesia Nomor 26 Tahun 2018 tentang Pelaksanaan kaidah pertambangan yang baik dan pengawasan pertambangan mineral dan batubara. Indonesia.

Pulungan, A.S.S. (2013) ‘Infeksi fungi mikoriza arbuskula pada akar tanaman tebu (Saccharum officinarum L.)’, Jurnal Biosains Unimed, 1(1), pp. 43–46.

Puspanti, A. (2013) ‘Kajian fitoremediasi sebagai salah satu pendukung kegiatan pengelolaan lahan pasca penambangan batubara’, in W. Gunawan, I. Yassir, and A. Puspanti (eds) Reklamasi Lahan Pasca Tambang: Aspek Kebijakan, Konservasi dan Teknologi. Balikpapan: Balai Penelitian Teknologi Konservasi Sumber Daya Alam, pp. 149–155.

Rajapakse, S. and Miller, J.C. (1992) ‘15 Methods for Studying Vesicular-arbuscular Mycorrhizal Root Colonization and Related Root Physical Properties’, Methods in Microbiology, 24, pp. 301–316. Available at: https://doi.org/10.1016/S0580-9517(08)70098-9.

Redecker, D., Schüßler, A., Stockinger, H., Stürmer, S.L., Morton, J.B. and Walker, C. (2013) ‘An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota)’, Mycorrhiza, 23(7), pp. 515–531. Available at: https://doi.org/10.1007/s00572-013-0486-y.

Säle, V., Aguilera, P., Laczko, E., Mäder, P., Berner, A., Zihlmann, U., van der Heijden, M.G.A. and Oehl, F. (2015) ‘Impact of conservation tillage and organic farming on the diversity of arbuscular mycorrhizal fungi’, Soil Biology and Biochemistry, 84, pp. 38–52. Available at: https://doi.org/10.1016/j.soilbio.2015.02.005.

Schüßler, A. and Walker, C. (2010) The glomeromycota: A species list with new families and new genera. Kew (GB): The Royal Botanic Garden Kew.

Setiadi, Y. and Setiawan, A. (2011) ‘Studi status fungi Mikoriza Arbuskula di areal rehabilitasi pasca penambangan nikel (Studi kasus PT INCO Tbk. Sorowako, Sulawesi Selatan)’, Jurnal Silvikultur Tropika, 03(01), pp. 88–95.

Shi, Z.Y., Zhang, L.Y., Li, X.L., Feng, G., Tian, C.Y. and Christie, P. (2007) ‘Diversity of arbuscular mycorrhizal fungi associated with desert ephemerals in plant communities of Junggar Basin, northwest China’, Applied Soil Ecology, 35(1), pp. 10–20. Available at: https://doi.org/10.1016/j.apsoil.2006.06.002.

Smith, S.E. and Read, D.J. (2008) Mycorrhizal symbiosis. 3rd edn. London: Elsivier.

Suharno, Sancayaningsih, R.P., Soetarto, E.S. and Kasiamdari, R.S. (2014) ‘Keberadaan fungi Mikoriza Arbuskula di kawasan tailing tambang emas Timika sebagai upaya rehabilitasi lahan ramah lingkungan’, Jurnal Manusia dan Lingkungan, 21(3), pp. 295–303.

Teixeira-Rios, T., Souza, R.G. de, Maia, L.C., Oehl, F. and Lima, C.E.P. (2013) ‘Arbuscular mycorrhizal fungi in a semi-arid, limestone mining-impacted area of Brazil’, Acta Botanica Brasilica, 27(4), pp. 688–693. Available at: https://doi.org/10.1590/S0102-33062013000400006.

Widodo (2011) ‘Kajian pemanfaatan lahan bekas tambang skala kecil untuk pertanian’, Jurnal Teknologi Mineral dan Batubara, 7(3), pp. 107–113.