EFFECTS OF TEMPERATURE AND NUTRIENT FEED ON THE PRODUCTION OF OXALIC ACID BY ASPERGILLUS NIGER
DOI:
https://doi.org/10.30556/imj.Vol14.No3.2011.482Keywords:
oxalic acid, Aspergillus niger, temperature, nutrient feed, batch and fed batch fermentationAbstract
Oxalic acid is an important organic material used for rare earth metal extraction precipitator, metallic equip- ment purificant and purification of industrial minerals. Aspergillus niger is known to be able to produce a high concentration of oxalic acid using glucose as carbon and energy sources. For further process optimiza- tion, submerged fermentation experiments were carried out to study the effect of temperature and nutrient feed on the production of oxalic acid from medium containing glucose 35g/L. An increase in temperature process from 25 to 30°C allowed the productivity to significantly increase from 75.50 to 81.06% of theoretical yield with a final oxalic acid concentration of 17.04 g/L reached after 9 days of process. When operating at more controlled fermentor with fed-batch system, both productivity and oxalic acid concentration were markedly improved (88.01% of theoretical yield and 19.52 g/L respectively). The later system gave excel- lent yield almost 90% of theoretical yield as a prerequisite of economical value.
References
Cameselle, C., J.T. Bohlmann, M.J. Nunez and J.M. Lema. 2003. Iron removal from kaolin: Compari- son between “in situ” and “two-stage” bioleching processes. Hydrometallurgy 68, p.97-105.
China Research and Intelligence Co., Ltd., 2009. Research report of Chinese oxalic acid industry. Http://www.marketresearch.com/product
Gharieb, M.M., 2000. Nutritional effects on oxalic acid production and solubilization of gypsum by As- pergillus niger. Mycological Research Vol. 104, Issue 05, May 2000.
Handayani, S. and Suratman, 2009. Production of oxalic acid by Aspergillus niger. Indonesian Min- ing Journal Vol. 12. No. 2, June 2009, p.85-89.
Handayani,S., 2004. Biobeneficiation of quartz us- ing Aspergillus niger. Buletin Bahan Galian In- dustry Vol. 8 No. 22. August 2004.
Kubicek, C.P., G. Schreferl-Kunar, W. Wohrer and M.Rohr. 1987. Evidence for a cytoplasmic path- way of oxalate biosynthesis in Aspergillus niger. Appl. Environ. Microbiol. 55, p.633-637.
Musial, I., W. Rymowicz and D. Witkowska, 2006. Ef- fect of span 20 concentration on oxalic acid pro- duction from post-refining fatty acids by Aspergil- lus niger SP. Chemical Papers Vol. 60, No. 5. October 2006.
Podgorski, W. and W. Lesniak, 2003. Biochemical method of oxalic acid production from beet mo- lasses. Chemical Papers 57 (6). p.408-412.
Ruijter, G.J.G., P.J.I. van de Vondervoort and J. Visser, 1999. Oxalic acid production by Aspergillus niger; an oxalate-non-producing mutant produces cit- ric acid at pH 5 and in the presence of manga- nese. Microbiology 145. p.2569-2576.
Rymowicz, W. and D. Lenart, 2004. Enhanced pro- duction of oxalic acid in Aspergillus niger by the addition of methanol. Electronic Journal of Pol- ish Agricultural Universities. Vol. 7 Issue 2.
Strasser, H., T. Pumpel, H. Brunner and F.Schinner. 1993. Improvement of the quality of quartz sand by means of microbial leaching of iron oxide. Arch. Geol. 16, p.103-107.
Styriakova, I. and I. Styriak, 2000. Iron removal from kaolins by bacterial leaching. Ceramics-Silicaty 44 (4) p.135 – 141.
Wang, N.S., 2002. Experiment no 4A and 9D : Glu- cose and sucrose assays by the dinitrosalicylic, Department of Chemical Engineering, Univer- sity of Maryland.
Wood, L., 2011. Research and markets: China’s ox- alic acid industry 2010-2012. Http:// www.researchandmarkets.com, accessed on September 20, 2011.
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